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首页> 外文OA文献 >Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
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Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning

机译：忍冬忍冬RAPD-SCAR标记的开发。通过改进的RAPD和DNA克隆进行（帽科）品种鉴定

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摘要

Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by an improved random amplified polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific L. japonica variety. The DNAs were extracted from fresh young leaves of different samples of L. japonica collected from Shenzhen, Yichang, Leshan, Emei and Loudi, China. The DNA materials were amplified using improved RAPD PCR. Different RAPD bands were excised, cloned and developed for stable sequence-characterized amplified region (SCAR) markers with different species. Two SCAR markers, JYH3-3 and JYH4-3, have been successfully cloned from improved RAPD fragments. The SCAR marker JYH3-3 was found specific for all of the L. japonica samples collected from the different regions, and another marker JYH 4-3 was strictly specific to the Shenzhen sample from Guangdong province, which is geographically distant from Hubei, Sichuan and Hunan Provinces (source of other L. japonica samples). The marker JYH3-3 was found as specific molecular marker for the identification of L. japonica, while JYH4-3 was found as molecular marker strictly specific for the Shenzhen sample. The developed SCAR markers might serve as more specific molecular markers for L. japonica variety authentication. The combination of improved RAPD analysis and SCAR marker development have resulted useful tools to study the genetic variety of any organism, which we have successfully applied here in L. japonica.de cualquier organismo, que hemos aplicado con éxito en L. japonica.
机译：物种内的遗传多样性是一个共同特征，在其生存和适应性中起着至关重要的作用，并且对于物种的识别和鉴定非常重要。忍冬（Lonicera japonica）是传统上使用的药用植物，最近已通过改进的随机扩增多态性DNA（RAPD）分析进行了遗传鉴定。在这项研究中，已经开发了基于这些RAPD片段的分子标记来鉴定特定的日本粳稻品种。该DNA提取自深圳，宜昌，乐山，峨眉和娄底的不同等级的日本粳稻样品的新鲜幼叶。使用改良的RAPD PCR扩增DNA材料。切除，克隆和开发不同的RAPD条带，以形成具有不同物种的稳定序列表征的扩增区（SCAR）标记。从改良的RAPD片段成功克隆了两个SCAR标记JYH3-3和JYH4-3。发现SCAR标记JYH3-3特异于从不同地区收集的所有粳稻样品，另一个标记JYH 4-3特异于广东省的深圳样品，该样品在地理上远离湖北，四川和四川。湖南省（其他粳稻样品的来源）。发现标记JYH3-3是鉴定日本粳稻的特异性分子标记，而发现JYH4-3是对深圳样品严格特异性的分子标记。所开发的SCAR标记可能用作日本粳稻品种鉴定的更具体的分子标记。改进的RAPD分析和SCAR标记开发相结合，已成为研究任何生物的遗传多样性的有用工具，我们已在这里成功地将其应用到了L. japonica.de cualquier生物体，ap aplicado conéxitoen L. japonica中。

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